Integrating DIY and Traditional Biochemical Laboratory Experiences into an Undergraduate Biophysics Course

Abstract

Many undergraduate physics majors will go directly to industry after graduation. Incorporating laboratory research into senior level courses facilitates the teaching of interdisciplinary skills such as designing experiments, careful measurement, and troubleshooting experimental difficulties. In addition to these skills hacking and making are also valuable for physics majors seeking employment directly after graduation. Two experiments were utilized to engage students in building these skills. In the first, traditional biochemical studies of phase behavior in lipid membranes was done with fluorescent probes. Fluorescence spectroscopy assays were used to measure main chain phase transitions in lipid bilayers and transbilayer flip-flop rates for phospholipid/cholesterol liposomes. Results from both assay experiments were compiled across teams of students. Each student was also responsible for producing a technical written lab report focusing on the experimental methods and results. In a second experience students attempted to build a Brewster Angle microscope using 3D printed parts and a simple cell phone camera. This project introduced students to CAD software and 3D printing.