Abstract
Chinese sour jujube (Ziziphus jujuba var. spinosa (Bunge) Hu ex H. F. Chow) is one of woody plant with the same origin of medicine and food. Specialized metabolites including flavonoids are mainly distributed in its active ingredient, but the composition and biosynthesis mechanism of flavonoids in different harvest times are still unclear. Hence, the accumulation of flavonoids in Chinese sour jujube fruits from five harvest times was investigated, using a combined metabolomics and transcriptomics approach in this study. And a total of 1539 differentially accumulated metabolites (DAMs) and 11592 differentially expressed genes (DEGs) were identified. The flavonoid, flavone, and flavanols biosynthtic pathways were significantly enriched in the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of DEGs and DAMs. Furthermore, a comprehensive regulatory network was built for flavonoids biosynthesis and it was discovered that one FLS gene, and one NAC transcription factors (TFs) with a high connection with flavonoids biosynthesis by weighted gene co-expression network analysis (WGCNA). Finally, RT-qPCR analysis of 15 flavonoid biosynthesis-related key genes validated the transcriptome sequencing data. This study provides a basis for further research and utilization of flavonoids in Chinese sour jujube and which is beneficial to the metabolic engineering of flavonoids.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
