Abstract
BackgroundMicroRNAs are important gene expression regulators in plants immune system. Aspergillus flavus is the most common causal agents of aflatoxin contamination in peanuts, but information on the function of miRNA in peanut-A. flavus interaction is lacking. In this study, the resistant cultivar (GT-C20) and susceptible cultivar (Tifrunner) were used to investigate regulatory roles of miRNAs in response to A. flavus growth.ResultsA total of 30 miRNAs, 447 genes and 21 potential miRNA/mRNA pairs were differentially expressed significantly when treated with A. flavus. A total of 62 miRNAs, 451 genes and 44 potential miRNA/mRNA pairs exhibited differential expression profiles between two peanut varieties. Gene Ontology (GO) analysis showed that metabolic-process related GO terms were enriched. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses further supported the GO results, in which many enriched pathways were related with biosynthesis and metabolism, such as biosynthesis of secondary metabolites and metabolic pathways. Correlation analysis of small RNA, transcriptome and degradome indicated that miR156/SPL pairs might regulate the accumulation of flavonoids in resistant and susceptible genotypes. The miR482/2118 family might regulate NBS-LRR gene which had the higher expression level in resistant genotype. These results provided useful information for further understanding the roles of miR156/157/SPL and miR482/2118/NBS-LRR pairs.ConclusionsIntegration analysis of the transcriptome, miRNAome and degradome of resistant and susceptible peanut varieties were performed in this study. The knowledge gained will help to understand the roles of miRNAs of peanut in response to A. flavus.
Highlights
MicroRNAs are important gene expression regulators in plants immune system
Peanut production is vulnerable to the threat of aflatoxin contamination, which is caused by the infection of Aspergillus flavus and Aspergillus parasiticus [2]
In two days after inoculation (DAI), hyphae were only observed in the seeds of Tifrunner, but not in GT-C20 treatment (GT)-C20 (Fig. 1)
Summary
Aspergillus flavus is the most common causal agents of aflatoxin contamination in peanuts, but information on the function of miRNA in peanut-A. flavus interaction is lacking. L), or groundnut, is one of the most important oil crops in the world. It is wildly planted in Asia, Africa, and North America. Peanut production is vulnerable to the threat of aflatoxin contamination, which is caused by the infection of Aspergillus flavus and Aspergillus parasiticus [2]. The aflatoxin was first revealed in 1960, when the aflatoxin-contaminated peanut caused the death of a large number of turkeys in UK. Peanut aflatoxin contamination is serious problem in China and limits the exportation of peanuts. Contamination can occur in the field, during harvest, or in storage and processing, and aflatoxin contamination is hard to prevent and control
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