Abstract
The label-free methods of proteomic combined with metabolomics were applied to explore the mechanisms of Cryptotanshinone (CPT) intervention in rats with acne. The model group consisted of rats given oleic acid (MC), then treated with CPT, while control groups did not receive treatment. The skin samples were significantly different between control, model and CPT-treated groups in hierarchical clustering dendrogram. Obvious separations of the skin metabolic profiles from the three groups were found through PCA scoring. In total, 231 and 189 differentially expressed proteins (DEPs) were identified in MC and CPT groups, respectively. By the KEGG analysis, five protein and metabolite pathways were found to be significantly altered. These played important roles in response to oleic acid-induced acne and drug treatment. CPT could negatively regulate glycolysis/gluconeogenesis and histidine metabolisms to decrease keratinocyte differentiation and improve excessive keratinization and cellular barrier function. CPT could down-regulate the IL-17 signaling pathway and regulate the acne-driven immune response of sebum cells. The biosynthesis of unsaturated fatty acids metabolism, glycerophospholipid metabolism and linoleic acid pathways could significantly alter sebum production and control sebaceous gland secretion after CPT treatment. The gap junction was up-regulated after CPT treatment and the skin barrier turned back to normal. Krt 14, Krt 16 and Krt 17 were significantly down-regulated, decreasing keratinization, while inflammatory cell infiltration was improved by down-regulation of Msn, up-regulation of linoleic acid and estrogen pathways after CPT treatment. These results propose action mechanisms for the use of CPT in acne, as a safe and potential new drug.
Highlights
Acne vulgaris has become one of the most common skin diseases (Oules et al, 2020) since more than 85% of teenagers and young adults have been affected worldwide (Kang et al, 2015)
model control (MC) rats were treated with CPT (Figure1 CPT) and showed skin tissue similar to the Blank control group (BC) group (Figure1 BC) with reduced keratinization
The MC group was significantly separated from both the BC and the CPT groups, and the skin samples in the BC group were closer to the CPT group
Summary
Acne vulgaris has become one of the most common skin diseases (Oules et al, 2020) since more than 85% of teenagers and young adults have been affected worldwide (Kang et al, 2015). Acne vulgaris is considered a chronic skin inflammation caused by pilosebaceous (Saurat, 2015), and sebaceous glands (SG) abnormally increased in the hair follicles (HF) (Li Z. et al, 2021). Mechanisms of Cryptotanshinone Intervene Acne growth, excessive hair follicle keratinization, skin bacteria colonization and skin inflammation (O’Neill and Gallo, 2018; Harper, 2020). It is often used to treat multiple chronic diseases, including angiocardiopathy, hyperlipidemia, acne vulgaris and chronic renal failure, with few side effects (Rahman et al, 2016; Zhang et al, 2019). The underlying mechanisms of the anti-acne effects of CPT have not been studied yet
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