Abstract
The possibilities of parallel lactic acid and biomass production in batch and fed-batch fermentation on distillery stillage from bioethanol production were studied. The highest lactic acid yield and productivity of 92.3% and 1.49gL(-1)h(-1) were achieved in batch fermentation with initial sugar concentration of 55gL(-1). A significant improvement of the process was achieved in fed-batch fermentation where the concentration of lactic acid was increased to 47.6% and volumetric productivity for 21% over the batch process. A high number of Lactobacillus rhamnosus ATCC 7469 viable cells of 10(9)CFUml(-1) was attained at the end of fed-batch fermentation. The survival of 92.9% of L. rhamnosus cells after 3h of incubation at pH 2.5 validated that the fermentation media remained after lactic acid removal could be used as a biomass-enriched animal feed thus making an additional value to the process.
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