Abstract
AbstractAn efficient pilot‐scale process was developed to produce nutraceutical products from fresh sea buckthorn (Hippophae rhamnoides) berries. Fresh berries were subjected to high‐pressure dewatering using a continuous screw press. The separated liquid phase containing 80–90% of pulp oil was clarified at 80 °C and centrifuged to obtain pulp oil, clear juice and sludge. The pulp oil yield was 2.7–2.8% of fresh berry weight with 66–70% extraction efficiency. The pulp oil was remarkably rich in carotenoids (2450–2810 mg kg−1), tocopherols (1409–1599 mg kg−1) and sterols (4096–4403 mg kg−1), with a characteristic fresh berry flavour and 16:1 as the major fatty acid (45.6–49.1%). The clear juice obtained was free from oil and contained high amounts of vitamin C (1683–1840 mg kg−1) and phytochemicals such as polyphenols (2392–2821 mg kg−1) and flavonoids (340–401 mg kg−1). Isorhamnetin (251–310 mg kg−1) was the major flavonoid in the juice, along with quercetin (77–81 mg kg−1) and kaempherol (12–16 mg kg−1). The juice was very acidic (pH 3), with high concentrations of organic acids (30.8–36.0 g kg−1). High‐performance liquid chromatography profiling of organic acids revealed quinic acid (18.1–19.9 g kg−1) as the major acid in the juice. The seeds in the pressed cake were separated and extracted for oil using supercritical CO2. Copyright © 2006 Society of Chemical Industry
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