Abstract
The potential use of a novel multichannel optical system towards fast and non-destructive bacteria identification and its application for environmental bacteria characterisation on the strain level is presented. It is the first attempt to use the proposed optical method to study various bacteria species (Gram-negative, Gram-positive) commonly present in the environment. The novel configuration of the optical system enables multichannel examination of bacterial colonies and provides additional functionality such as registration of two-dimensional (2D) distribution of monochromatic transmission coefficient of examined colonies, what can be used as a novel optical signature for bacteria characterization. Performed statistical analysis indicates that it is possible to identify representatives of environmental soil bacteria on the species level with the 98.51% accuracy and in case of two strains of Rahnella aquatilis bacteria on the strain level with the 98.8% accuracy. The proposed method is an alternative to the currently used preliminary bacteria examination in environment safety control with the advantage of being fast, reliable, non-destructive and requiring minimal sample preparation.
Highlights
Bacteria identification is an essential foundation of the microbiological medical and veterinary diagnosis, but it is important in such areas, as food safety control, monitoring environmental contamination and in agriculture
50 Fresnel diffraction patterns with a unique spatial distribution of the diffracted light intensity were generated for each class of bacteria species/strains, giving a total number of 450 registered patterns
The registered diffraction patterns exhibit a highly repeatable spatial distribution of the diffracted light intensity, what confirms the species-/straindependence of bacterial colonies Fresnel patterns for standardised incubation conditions
Summary
Bacteria identification is an essential foundation of the microbiological medical and veterinary diagnosis, but it is important in such areas, as food safety control, monitoring environmental contamination and in agriculture. Many methods based on different detection principles are commonly available for identification of environmental bacteria. The most widely used techniques include biochemical, molecular and mass spectrometric methods [1,2,3,4,5,6,7]. Alternative methods use fluorescence in situ hybridization (FISH) technique, which is cytogenetic method utilizing fluorescence probes [10], biochemical techniques, such as BIOLOG, or novel culturing methods [11] or mass spectroscopy using MALDI – TOF (Matrix - Assisted Laser Desorption - Ionization - Time Of Flight) [3,4,12] that provide new insights of the microbiological diversity in the environment
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
