Integrated Isoform Sequencing and Dynamic Transcriptome Analysis Reveals Diverse Transcripts Responsible for Low Temperature Stress at Anther Meiosis Stage in Rice.

Jingguo Wang,Yan Jia,Xinpeng Wang,Zhaojun Qu,Hualong Liu,Detang Zou,Hongyang Chen,Yuyang Duan,Hongwei Zhao,Hongliang Zheng

doi:10.3389/fpls.2021.795834

Jingguo Wang, Yan Jia + Show 8 more

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https://doi.org/10.3389/fpls.2021.795834

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Journal: Frontiers in plant science	Publication Date: Dec 17, 2021
Citations: 5	License type: CC BY 4.0

Affiliation: Northeast Agricultural University

Abstract

Low temperatures stress is one of the important factors limiting rice yield, especially during rice anther development, and can cause pollen sterility and decrease grain yield. In our study, low-temperature stress decreased pollen viability and spikelet fertility by affecting the sugar, nitrogen and amino acid contents of anthers. We performed RNA-seq and ISO-seq experiments to study the genome-wide transcript expression profiles in low-temperature anthers. A total of 4,859 differentially expressed transcripts were detected between the low-temperature and control groups. Gene ontology enrichment analysis revealed significant terms related to cold tolerance. Hexokinase and glutamate decarboxylase participating in starch and sucrose metabolism may play important roles in the response to cold stress. Using weighted gene co-expression network analysis, nine hub transcripts were found that could improve cold tolerance throughout the meiosis period of rice: Os02t0219000-01 (interferon-related developmental regulator protein), Os01t0218350-00 (tetratricopeptide repeat-containing thioredoxin), Os08t0197700-00 (luminal-binding protein 5), Os11t0200000-01 (histone deacetylase 19), Os03t0758700-01 (WD40 repeat domain-containing protein), Os06t0220500-01 (7-deoxyloganetin glucosyltransferase), Pacbio.T01382 (sucrose synthase 1), Os01t0172400-01 (phospholipase D alpha 1), and Os01t0261200-01 (NAC domain-containing protein 74). In the PPI network, the protein minichromosome maintenance 4 (MCM4) may play an important role in DNA replication induced by cold stress.

Highlights

Rice (Oryza sativa L.) is a staple food for over 60% of the Chinese population (Zhu et al, 2010)
Under LT stress, concentrations of 15 amino acids increased, especially Asp, Glu, Arg, and Tyr (which increased by an average of 2.8, 2.0, 2.2, and 2.3 times, FIGURE 1 | Effect of low temperature on the sucrose (A), fructose (B), glucose (C), soluble sugar (D), soluble starch (E), and total carbon (F) content in the anther of rice
LT inhibits the expression of OsINV4 and OsMST8 in the tapetum, leading to disruption of starch formation during anther development (Oliver et al, 2005; Mamun et al, 2006).we speculate that the decrease in rice anther enzyme activity under longterm LT stress leads to the interruption of starch synthesis and at the same time, causes more carbon skeletons to participate in nitrogen metabolism, which explains the reason for the increase in nitrogen content (Asthir et al, 2013)

Summary

Introduction

Rice (Oryza sativa L.) is a staple food for over 60% of the Chinese population (Zhu et al, 2010). LT stress during this period disrupts the callose wall formed in tapetal cells and limits the provision of nutrition to young microspores and developing pollen grains, leading to male sterility (Oliver et al, 2005; Mamun et al, 2006, 2010; Oda et al, 2010; Fahad et al, 2018; Zhang et al, 2021). Previous research found that LT stress increased abscisic acid (ABA) levels, decreased gibberellic acid (GA) levels in the tapetum, and decreased the activity of cell wallbound acid invertase, thereby inducing tapetum hypertrophy and delaying programmed cell death (PCD), leading to sucrose accumulation in the tapetum, and disrupted hexose production and starch formation in the pollen grains, and resulting in a decline in pollen viability (Oliver et al, 2005, 2007; Li et al, 2006; Zhang et al, 2021)

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