Integrated immunoassay-based broad detection of multi-class mycotoxins

Shupeng Yang,Xiujuan Yi,Xin Mao,Yunguo Liu,Suxia Zhang,Yanshen Li

doi:10.1080/09540105.2018.1424121

Abstract

ABSTRACT Europium (Eu3+) and horse radish peroxidase (HRP) are highly promising biosensors due to its excellent physical and chemical properties. The two biosensors are widely applied in time-resolved fluoroimmunoassay (TRFIA) and chemiluminescence enzyme-linked immunosorbent assay, respectively. In this work, the two technologies were integrated with parallel input and parallel output (PI–PO) characters. Eu3+ was adopted as probe I in TRFIA for deoxynivalenol and de-epoxyldexoylnivalenol with a limit of detection (LOD) of 4.74 and 4.56 ng/mL, while HRP was performed as probe II in CL-ELISA for FB1 with an LOD of 0.088 ng/mL. Compared to the traditional individual assay, this method exhibited fast and broad detection characteristics by integrating two immunoassays into one.

Highlights

Mycotoxins, such as Fumonisins B1 (FB1), deoxynivalenol (DON), and its major metabolite de-epoxyldexoylnivalenol (DOM-1), are mainly produced by fungi when storage of cereals is inadequate (He et al, 2017; Kong, Xie, Liu, Song, & Kuang, 2017)
Eu3+ was adopted as probe I in time-resolved fluoroimmunoassay (TRFIA) for deoxynivalenol and de-epoxyldexoylnivalenol with a limit of detection (LOD) of 4.74 and 4.56 ng/mL, while horse radish peroxidase (HRP) was performed as probe II in CL-ELISA for FB1 with an LOD of 0.088 ng/mL
KEYWORDS parallel input and parallel output (PI–PO); multi-classes analysis; integrated immunoassay; biosensor

Summary

Introduction

Mycotoxins, such as Fumonisins B1 (FB1), deoxynivalenol (DON), and its major metabolite de-epoxyldexoylnivalenol (DOM-1), are mainly produced by fungi when storage of cereals (structure shown in Figure 1) is inadequate (He et al, 2017; Kong, Xie, Liu, Song, & Kuang, 2017). These mycotoxins can induce various hazardous effects on animals and human beings even at a very low level (Bottalico & Perrone, 2002; Juan, Raiola, Mañes, & Ritieni, 2014). A number of methodologies have been reported for mycotoxins’ detection, such as gas chromatography coupled to mass spectrometry (Tacke & Casper, 1996) and liquid chromatography tandem mass spectrometry

Methods

Results

Conclusion