Integrated crop practices management stimulates soil microbiome for Verticillium wilt suppression

Abstract

Understanding the components that shape the rhizosphere community is vital for sustainable disease management. This study evaluated an integrated Verticillium wilt management in eggplant and its influence on the soil microbiome. Six treatments; Self-rooted (control; CLA) and sole grafted (CLB) eggplants, Brassica + Self-rooted plant (BrA), and Brassica + Grafted plants (BrB), with Biochar (10 t/ha) + Brassica + Self-rooted plant (BBrA) and Biochar (10 t/ha) + Brassica + Grafted Plant (BBrB) were used. Soil microbiome was characterized using high-throughput sequencing. The grafted treatments significantly reduced the Verticillium abundance, disease index and improved the yield of eggplant compared with CLA (18.13 t/ha), with BBrB (41.54 t/ha) as the best treatment. Results showed that treatments CLB, BrB, and BBrB stimulated more beneficial microbes, especially Arthrobacter, Bacillus, and Sphingomonas for bacteria; and Mortierella, Tausonia, and Chaetomium for fungi. Treatment BBrB was biomarked by phylum Chloroflexi (o_SBR1031), Acidobacteria, Planctomycetes, and Patescibacteria, but only Chloroflexi (o_SBR1031) was found in BrB, and none of them in CLB and treatment BBrB also contained more biomarkers than other treatments. Similarly, the Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis revealed that treatment BBrB contained more genes (17.5%) regulating disease resistance followed by BrB (17.3%) and CLB (16.6%) treatments. In conclusion, grafting with Brassica (biofumigation) under a biochar regime could reduce eggplant Verticillium wilt. This study expands knowledge on how soil microbiota can be enhanced using integrated disease management practices to exploit sustainable food production.