Abstract
The replacement of expensive components in microbial growth media with pretreated lignocellulosic waste component to increase the product spectrum and add value to the bioproducts has been encouraged to achieve sustainable and feasible utilization of waste biomass as per the biorefinery approach. This study demonstrates an integrated biorefinery approach towards utilization of sugarcane bagasse and biomass of Mucor circinelloides ZSKP. A maximum reducing sugar recovery of 80.67 g/l was achieved after combining pretreatment with saccharification. A low temperature, glycerol, and ammonium phosphate pretreatment method was established, where glycerol pretreatment conditions were reduced from 250 to 150 °C and from 120 to 45 min. The ammonium phosphate-containing hydrolysate yielded 12.89 g/l of fungal biomass after fermentation to add to 20.8 g lignin from the delignification step. The biomass production was further improved to 17.69 g/l after supplementation with corn steep solids and mineral salts. The fermentation process also yielded 2.36 g/l chitosan and 4.9 g/l of lipids after extraction from the oleaginous fungus. The lignin infused glycerol plasticized chitosan biocomposite plastic had a 100% improvement in thermogravimetric properties with almost 50% more energy needed to increase the temperature of the material when compared to glycerol only plasticized biocomposite. The fungal chitosan showed antimicrobial properties and was effective as a preservative spray for fresh tomatoes and apples extending their shelf life to at least 14 and 18 days, respectively. This study therefore demonstrated that a novel two-step pretreatment process could be environmentally beneficial and yielded multiple products for biotechnological applications.
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