Integrated assessment of the acclimation capacity of the marine bivalve Crenomytilus grayanus under naturally highly contaminated conditions: Subcellular distribution of trace metals and structural alterations of nephrocytes

Abstract

The aim of our work was to assess whether the cellular processes in the nephrocytes of the long-lived mussel Crenomytilus grayanus tend to acclimation or destruction under trace metal contamination. Mussels were collected from three sites in the north-western Pacific Ocean: reference site, upwelling site, and a site highly contaminated with trace metals. Concentration, subcellular distribution of trace metals (Cd, Cu, Zn, and Pb) in the mussel kidneys, and ultrastructural alterations of the nephrocytes were studied. To assess the total load of accumulated trace metals, the total concentration coefficient (∑СС) was determined.In the kidneys of the reference C. grayanus, trace metals were eliminated from cell metabolism mainly by lysosomal granules or residue bodies. Under high levels of contamination, the defense mechanisms of C. grayanus are practically suppressed (no metallothionein-like protein peak, decreased content of granules) by the total effect of accumulated pollutants that leads to the destruction of cellular structures. Under natural conditions (upwelling site), increased accumulation of trace metals in the mussel kidneys did not lead to an increase in the number or size of lysosomal granules. However, abnormal high Cd accumulation in the kidneys caused the synthesis of high levels of metallothionein-like proteins that sequester most of the studied trace metals. To quickly lower the metal levels in nephrocytes under these conditions, a unique long-term acclimatory response — apocrine-like secretion in nephrocytes, which provides rapid elimination of me—MTLP complexes from the cell arose. Thus, our integrated study of the subcellular distribution of trace metals and ultrastructural alterations in nephrocytes allowed us to characterize the features of the structural and functional alterations in mussel cells under the field conditions tested.