Abstract
RNA-binding proteins (RBPs) play significant roles in various cancer types. However, the functions of RBPs have not been clarified in renal papillary cell carcinoma (pRCC). In this study, we identified 31 downregulated and 89 upregulated differentially expressed RBPs on the basis of the cancer genome atlas (TCGA) database and performed functional enrichment analyses. Subsequently, through univariate Cox, random survival forest, and multivariate Cox regression analysis, six RBPs of SNRPN, RRS1, INTS8, RBPMS2, IGF2BP3, and PIH1D2 were screened out, and the prognostic model was then established. Further analyses revealed that the high-risk group had poor overall survival. The area under the curve values were 0.87 and 0.75 at 3 years and 0.78 and 0.69 at 5 years in the training set and test set, respectively. We then plotted a nomogram on the basis of the six RBPs and tumor stage with the substantiation in the TCGA cohort. Moreover, we selected two intersectant RBPs and evaluate their biological effects by GSEA and predicted three drugs, including STOCK1N-28457, pyrimethamine, and trapidil by using the Connectivity Map. Our research provided a novel insight into pRCC and improved the determination of prognosis and individualized therapeutic strategies.
Highlights
Renal cell carcinoma (RCC), which accounts for 3% of adult malignancies, is a fatal malignancy of the urinary system (Huang et al, 2017)
RNA sequencing data containing 32 normal samples and 289 tumor samples of papillary cell carcinoma (pRCC) and clinical data were downloaded from the the cancer genome atlas (TCGA) database
The expression of Differentially Expressed Genes (DEGs) was plotted in a heatmap for visualization (Figures 2A,B). 285 tumor samples were selected after filtering out samples which were lack of clinical survival information
Summary
Renal cell carcinoma (RCC), which accounts for 3% of adult malignancies, is a fatal malignancy of the urinary system (Huang et al, 2017). RCC consists of three subtypes: renal clear cell carcinoma (ccRCC), renal papillary cell carcinoma (pRCC), and renal chromophobe cell carcinoma (chRCC) (Tabibu et al, 2019). CcRCC constitutes 70% of all RCC cases, whereas pRCC is the second common subtype of RCC constituting 15% (Al Ahmad et al, 2019). PRCC is considered as more inert than ccRCC. Advanced cases of pRCC have metastatic potential, which are more. RBPs in Renal Papillary Carcinoma lethal than ccRCC (Kaldany et al, 2019). A comprehensive analysis of vital genes in pRCC tumorigenesis is typically necessary to evaluate the individual prognosis, determine the therapeutic target, and predict potential drugs for patients with poor prognosis
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