Abstract
BackgroundDNA methylation, a biochemical modification of cytosine, has an important role in lipid metabolism. Fatty liver hemorrhagic syndrome (FLHS) is a serious disease and is tightly linked to lipid homeostasis. Herein, we compared the methylome and transcriptome of chickens with and without FLHS.ResultsWe found genome-wide dysregulated DNA methylation pattern in which regions up- and down-stream of gene body were hypo-methylated in chickens with FLHS. A total of 4155 differentially methylated genes and 1389 differentially expressed genes were identified. Genes were focused when a negative relationship between mRNA expression and DNA methylation in promoter and gene body were detected. Based on pathway enrichment analysis, we found expression of genes related to lipogenesis and oxygenolysis (e.g., PPAR signaling pathway, fatty acid biosynthesis, and fatty acid elongation) to be up-regulated with associated down-regulated DNA methylation. In contrast, genes related to cellular junction and communication pathways (e.g., vascular smooth muscle contraction, phosphatidylinositol signaling system, and gap junction) were inhibited and with associated up-regulation of DNA methylation.ConclusionsIn the current study, we provide a genome-wide scale landscape of DNA methylation and gene expression. The hepatic hypo-methylation feature has been identified with FLHS chickens. By integrated analysis, the results strongly suggest that increased lipid accumulation and hepatocyte rupture are central pathways that are regulated by DNA methylation in chickens with FLHS.
Highlights
DNA methylation, a biochemical modification of cytosine, has an important role in lipid metabolism
Comparison of DNA methylome profiles of chickens with and without Fatty liver hemorrhagic syndrome (FLHS) Hepatic DNA methylomes of chickens with and without FLHS were compared to determine whether hepatic lipid metabolism was regulated by methylation changes
The hepatic Cytosine-phosphate bond-guanine (CpG) (C represents cytosine and G represents guanine, while p represents phosphate bond between nucleotides) methylation levels of FLHS were lower in regions up- and down-stream of gene bodies, while it’s not identical to that in the gene body, the methylation difference in the gene body was relative small (Fig. 1b)
Summary
DNA methylation, a biochemical modification of cytosine, has an important role in lipid metabolism. Fatty liver hemorrhagic syndrome (FLHS) is a serious disease and is tightly linked to lipid homeostasis. We compared the methylome and transcriptome of chickens with and without FLHS. Fatty liver hemorrhagic syndrome (FLHS) is a serious disease, which is characterized by massive lipid accretion and hemorrhagic spots of the liver [1]. The. FLHS is tightly linked to lipid homeostasis, with disorders of synthesis, transport, and oxygenolysis [6]. Individuals with FLHS have elevated lipid metabolism, dominated by an anabolic process. With increased (2021) 22:8 triglyceride (TG) deposition in hepatocytes, enlarged hepatocytes and histological injury are observed [7]. The disappeared cellular boundaries and destroyed cellular junction are discovered, and the impaired hepatocyte structure is observed distinctly [8, 9]
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