Abstract
Selenium deficiency is closely related with various type of cardiovascular disease. However, the miRNA-mRNA regulatory network in Selenium deficiency related cardiac change remains to be understand. In the present study, a reliable Selenium deficiency rat model was established and confirmed by pathological and biochemical examination. The mRNA and miRNA expression profiles were conducted by microarray technology. Gene Ontology (GO) Analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway Analysis was performed to investigate the function of targeted genes, and the relationship between miRNA and mRNA was studied by network analysis. A total of 4931 mRNAs and 119 miRNAs was differentially expressed between any two groups (control group, low-selenium group and selenium supplementation group). GO and KEGG pathway analysis of selected miRNAs target genes found that selenium deficiency was related to several different biological processes. Furthermore, a miRNA-mRNA regulatory network was conducted to illustrate the interaction of miRNAs and these targeted genes. In conclusion, our present study provides a new insight that potential molecular mechanism of Selenium deficiency was a multiply miRNAs and mRNA caused biological change.
Highlights
Selenium is an essential trace element in mammals
HE staining revealed that histological changes of the rat hearts caused by selenium deficiency (Fig. 3)
Selenium-deficiency can cause a variety of cardiovascular diseases, most of them are induced by oxidative damage and inflammation[20]
Summary
Selenium is an essential trace element in mammals It plays important role in antioxidant, immune system, free radical scavenging and a series of crucial biological process[1]. An Integrated analysis of miRNA and mRNA expression make it possible to construct miRNA-mRNA interactive network, which help us further explore the relationship between cardiovascular disease induced by selenium deficiency and miRNA regulation. High-throughput gene chip technology has been used to explore the differential expression of miRNAs and mRNAs and performed a profiling of miRNA expression in selenium deficient rats. Comprehensive analysis was conducted for the first time to evaluate the functional miRNA-mRNA interactive network of selenium deficiency. By integrated analysis of miRNA and mRNA expression profiles, our study elucidated the potential biological and molecular mechanism of cardiac dysfunction in selenium deficient rat models
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