Abstract
DNA methylation has been demonstrated to play significant roles in the etiology and pathogenesis of head and neck squamous cell carcinoma (HNSCC). In the present study, methylation microarray dataset (GSE87053) and gene expression microarray dataset (GSE23558) were downloaded from GEO database and analyzed through R language. A total of 255 hypermethylated-downregulated genes and 114 hypomethylated-upregulated genes were finally identified. Functional enrichment analyses were performed and a comprehensive protein–protein interaction (PPI) network was constructed. Subsequently, the top ten hub genes selected by Cytoscape software were subjected to further analyses. It was illustrated that the expression level of CSF2, CTLA4, ETS1, PIK3CD, and CFTR was intimately associated with HNSCC. Survival analysis suggested that CTLA4 and FGFR2 could serve as effective independent prognostic biomarkers for HNSCC patients. Overall, our study lay a groundwork for further investigation into the underlying molecular mechanisms in HNSCC carcinogenesis, providing potential biomarkers and therapeutic targets for HNSCC.
Highlights
Head and neck squamous cell carcinoma (HNSCC) is a prevalent malignancy with high morbidity and mortality, causing approximately 300,000 deaths each year all over the world [1]
It is widely acknowledged that alcohol and tobacco consumption are the two most important risk factors for HNSCC pathogenesis while human papillomavirus (HPV) infection has increasingly been recognized as an essential etiological factor [2]
We examined the relationship of hub genes expression with the survival of HNSCC patients based on the the cancer genome atlas (TCGA) data in UCSC Xena
Summary
Head and neck squamous cell carcinoma (HNSCC) is a prevalent malignancy with high morbidity and mortality, causing approximately 300,000 deaths each year all over the world [1]. Traditional clinical treatment methods for HNSCC included ablative surgery, chemotherapy, and radiotherapy while novel therapeutic measures such as anti-EGFR biotherapy have been developed in recent years. In spite of numerous modalities conducted on therapy methods, the life quality of HNSCC patients was not remarkably improved and the survival rate desperately remains at 50% [3]. This outcome may result from the high metastasis and recurrence rates of HNSCC and the delayed detection and diagnosis due to the asymptomatic nature of the early stage of this disease.
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