Abstract

The culture chamber consists of two metal plates held together by four short screws, a thin (film-type) electrical heating unit, a silicone rubber gasket and two cover slips. The order of assembly is bottom plate, heating element, first cover slip, gasket, second cover slip, and top plate. Syringe needles, one containing a thermistor, and others for supply and removal of fluid are inserted into the chamber through the gasket. Temperature is controlled by electrical connections through a Thermistemp temperature controller.

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