Abstract
Our purpose is to determine whether cumulus cells enclosed around the oocyte during vitrification process have any beneficial effects on oocyte metabolism and developmental competencies. Basic animal study to evaluate developmental competencies of MII oocyte after cryopreservation with or without cumulus cells. We superovulated the mice with PMSG and HCG, then collected MII oocytes 14 hours after HCG injection. The collected oocytes were divided into vitrification-denuded oocyte (V-DO) group, vitrification-cumulus oocyte complex (V-COC) group, and control group. Each group included 50 oocytes for study. We used 15% dimethyl sulfoxmide plus 15% ethylene glycol as cryoprotectant and followed the standard vitrification procedures. The vitrified oocytes were stored in liquid nitrogen for at least 24 hours before warming, then cultured in 37OC, 5% CO2 incubator for 2 hours for recovering. We compared oocyte survival rates, Adenosine Triphosphate (ATP) contents, piezo-intracyplasmic sperm injection (piezo-ICSI) fertilization rates, and blastocyst formation rates among all three groups. Our results showed that both V-DO and V-COC groups have comparable oocyte survival rates after cryopreservation. Piezo-ICSI fertilization rates had no significant differences among V-DO and V-COC group, however, fertilization rates in both vitrification groups were lower than the rate in control group. COC group had higher ATP content and blastocyst formation rate as compared to the results from DO group.Table 1Comparison.GroupMIISurvivalATP Content (pmole/oocyte)FertilizationBlastocystV-DO5046 (92.0%)0.082 +/- 0.01*42 (91.3%)26 (61.9%)∗A significant difference: P less than 0.05.V-COC5047 (94.0%)0.110 +/- 0.0143 (91.5%)32 (74.4%)Control500.158 +/- 0.0147 (94.0%)39 (83.0%)* A significant difference: P less than 0.05. Open table in a new tab We conclude that oocytes with intact cumulus cells have better intracellular energy profiles and developmental competencies when undergoes vitritrification/warming procedures as compared with denuded oocytes.
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