Abstract
ObjectiveThe aim of this study was to compare the difference between insulin-producing cells (IPCs) and normal human pancreatic beta cells both in physiological function and morphological features in cellular level.MethodsThe levels of insulin secretion were measured by enzyme-linked immunosorbent assay. The insulin gene expression was determined by real-time quantitative polymerase chain reaction. The morphological features were detected by atomic force microscopy (AFM) and laser confocal scanning microscopy.ResultsIPCs and normal human pancreatic beta cells were similar to each other under the observation in AFM with the porous structure features in the cytoplasm. Both number of membrane particle size and average roughness of normal human beta cells were higher than those of IPCs.ConclusionsOur results firstly revealed that the cellular ultrastructure of IPCs was closer to that of normal human pancreatic beta cells, but they still could not mimic the physiological regulation of insulin secretion performed by pancreatic beta cells.
Highlights
Diabetes is caused by absolute or relatively insufficient insulin secretion
We found that Insulin-producing cells (IPCs) had a similar distribution of membrane proteins to normal pancreatic beta cells, they still could not mimic the physiological regulation of insulin secretion performed by normal pancreatic beta cells
The second passage of human adipose-derived stem cells (hADSCs) expanded rapidly and developed a uniform morphology that resembled that of fibroblasts
Summary
Diabetes is caused by absolute or relatively insufficient insulin secretion. Hitherto, there is no cure for diabetes. Insulin-producing cells (IPCs) from pluripotent stem cells offer the potential to treat diabetes by providing a source for injured pancreatic beta cells without the limitations of current therapeutic modalities. Differences in cell morphology can likely reveal the reason why there is great difference in cellular function. We compared the differences in morphology and function between normal human pancreatic beta cells and IPCs derived from human adipose-derived stem cells (hADSCs). We found that IPCs had a similar distribution of membrane proteins to normal pancreatic beta cells, they still could not mimic the physiological regulation of insulin secretion performed by normal pancreatic beta cells. We propose that the difference in physiological function between these two kinds of cells is due to the difference in the nanostructure of their cell membranes
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
