Abstract
Isolation of pure beta cells of the rat pancreas was achieved employing counterflow sedimentation technique (CST) followed by density gradient centrifugation technique (DGCT). The proportion of non-endocrine cells to beta cells was minimal (1 acinar cell in 296 beta cells, and 1 duct cell in 300 beta cells) with total absence of alpha and delta cells. Oxidation of D-(U- 14C) glucose to 14CO 2 by the isolated beta cells was linear to time. Glucagon (1, 5, or 10 nM) or arginine (1, 5 or 10 mM) produced concentration dependent insulin secretion. Thus, a highly purified preparation of isolated beta cells of rat pancreas could be obtained with excellent morphologic, metabolic and functional integrity.
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