Abstract
This paper presents the FTIR spectral study of saliva of normal and diseased (diabetes) and its use in differentiating those pregnant women on insulin therapy. FTIR saliva spectra were recorded over the region 4000-400 cm -1 on a Spectrum one Perkin Elmer FTIR spectrometer. Saliva samples were collected from 20 volunteers in each set of age group 25 to 35. From the spectral study, the absorbance values of the diseased and normal saliva samples were compared. The spectral differences of intensity ratio parameters were introduced and the results were discussed. In order to find the efficacy, the absorption values of the specific bands of the spectra of normal and diabetic pregnant women were compared and the internal ratio parameter R 1 ( I 1653 /I 1410 ), R 2 (I 2931 /I 547 ), R 3 (I 2931 /I 1410 ), R 4 (I 2931 /I 3292 ),R 5 (I 3292 /I 547 ) were calculated , and the result observed for diabetic pregnant women after insulin therapy (i.e., 3 hrs and 7 days) was almost similar to that of the normal pregnant women. Besides, the intensity of absorption peaks of the diabetic pregnant women before administration of insulin, after intake of insulin (3hrs) and after insulin therapy (7 days) were considered in which the intensity ratio parameters R 1 ( I 1544 /I 1075 ), R 2 (I 1544 /I 1224 ), R 3 (I 2931 /I 1075 ), R 4 (I 3292 /I 1075 ), R 5 (I 3292 /I 1224 ), were calculated. The observed absorbance values increased in diabetic pregnant women initially, but slightly decreased after insulin therapy 3 hrs and further decreased after 7 days compared to normal pregnant women. Thus, striking spectral differences (in terms of intensity value) observed between saliva of normal, diseased subjects when analysed after insulin therapy. The comparison of these values showed that the spectra of normal and diabetic pregnant women before and after therapy are different and the vibrational analyses were carried out. It is concluded that FTIR may be applied to indicate the changes in the salivary pattern of the diabetic pregnant women with insulin therapy.
Highlights
The greatest challenge of salivary diagnostics is to requirements for the growth of the fetus and the identify disease diagnostic markers and successfully preparation of the mother for delivery
FTIR spectroscopy as a bio diagnostic tool to predict changes occur during pregnancy
A representative FTIR absorption spectrum of saliva sample of normal pregnant women is shown in Fig
Summary
The greatest challenge of salivary diagnostics is to requirements for the growth of the fetus and the identify disease diagnostic markers and successfully preparation of the mother for delivery. Glucose is the main stimulus for insulin secretion, but the pancreas releases insulin in response to elevated blood levels of amino acids or when signaled by the parasympathetic (vagal) nervous system In this the saliva samples are collected from diabetic pregnant women of 1st, 2nd and 3rd trimester and FTIR analysis were carried out. The present work is attempted in the study of changes in salivary pattern in normal and diabetic pregnant women and the response in change in saliva on insulin therapy (after 3 hrs and after 7 days) are compared by using FTIR spectroscopy both qualitatively and quantitatively. The samples were collected from normal and diabetic pregnant women (after excluding other diseases) and the response in change in saliva of insulin therapy patients have been considered and chosen for the analysis.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
