INSULIN RESISTANCE ALTER THE LEVELS OF IL-4, IL-5 AND IL-13 IN TYPE 2 DIABETES MELLITUS WITH LESS THAN 5 YEARS OF DURATION

Abstract

The harmonious biochemical processes in a healthy individual are due to the balance between essential molecules that are necessary for the maintenance of physiology of the living system. These include energy bio-molecules, co-factors for oxidation to attain energy expenditure, regulatory hormones, and protective immune system. Objectives: Identifying predictive factors for 2DM will be benecial to developing effective prevention and early detection of the disease. Therefore, the novelty of this study is to evaluate whether inammatory markers are different in individuals with versus without T2DM. To assess the effect of glucose, insulin, Homeostasis metabolic assessment-estimated insulin resistance (HOMA-IR), lipids on cytokine variables in type 2DM subjects. Materials& Methods: Two hundred and thirty individuals are recruited in to this present study after the approval from Institutional ethical committee for the present study. The study was conducted in the Department of Biochemistry, Malwanchal university, Indore, India. Age & sex matched one hundred and fteen human non-2DM individuals were taken into healthy control group. One hundred and fteen subjects, on treatment for 2DM were included in second group.Results: On comparison of age, Tc, and Ldl parameters between 2DM and control group subjects showed insignicant differences, whereas parameters of FBS (t=12.24; df=228; P<0.001) HbA1c (t=16.40; df=228; P<0.05) and insulin mean levels (t=2.70; df=228; P<0.05) showed a signicant difference. Decrease in the levels of serum IL-4 was observed in 2DM subjects when compared with healthy control subjects. On the contrary, we observed increase in the values of IL-5 and IL-13 in 2DM subjects when compared with healthy control subjects. In case of IL-10 and IL-12 values, we observed no signicance when compared between the group subjects. Conclusion: The present study concludes that there is association of IL-4, IL-5 and IL-13 with relation to 2DM. These cytokines can be used as biomarkers for early identication and diagnosis of secondary complications in 2DM individuals.