Insulin mimetic lanostane triterpenes from the cultivated mushroom Ganoderma orbiforme

Abstract

• Three triterpenes derivatives were isolated from the cultivated fruiting bodies of Ganoderma orbiforme . • The triterpenes induced glucose uptake in a zebrafish-based assay. • The triterpenes also showed an inhibitory effect on nitric oxide production in LPS-induced BV-2 microglia cells. Two new lanostane triterpenes, ganorbifoins A and B ( 1 - 2 ), together with the known compound (25S,3′S)-(+)-12α-hydroxy-3α-(3′-hydroxy-4′-methoxycarbonyl-3′-methylbutiryloxy)-24-methyllanosta-824-(31)-dien-26-oic acid ( 3 ) were isolated from the cultivated fruiting bodies of Ganoderma orbiforme . The structures of isolates were determined by extensive analysis of NMR and HRESIMS. All compounds induced glucose uptake in zebrafish-based system at the concentration of 10 μM. And the best performing compound is ganorbifoin A. Compounds 2 and 3 exhibited an inhibitory effect on nitric oxide production in LPS-induced BV-2 microglia cells at the concentration of 20 μ M.