Abstract
Insulin, an old but still actual molecule, has many roles in various cellular processes including bone formation and also angiogenesis. Insulin effects on different cell types were investigated, and we intended to check its effect on dental pulp cells (DPC) during osteoblast differentiation. First, bone differentiation ability of isolated dental pulp cells was assessed by alizarin red (AR) staining. Insulin increased dental pulp cell proliferation after 25 hours of culture. It increased mRNA expression of osteogenic markers such as Col1, RunX2, ALP, Osc, Mef2C and expression of genes involved in TGF b pathway such as Smad3, TSP1, VEGF at different time points.
Highlights
Insulin is an anabolic molecule secreted by the beta cells of pancreas Langerhans islets
Calcium deposition was checked after 3 weeks of dental pulp cell culture in osteomedium or basal medium
There was information that insulin enhances TGFb receptors in the cell membrane by their translocation, but in this study, we showed that 200 ng/ml concentration of insulin significantly enhances TGFbR1, and TGFb, TGFb2 in mRNA level during osteodifferentiation of DPSC at 7 days of culture
Summary
Insulin is an anabolic molecule secreted by the beta cells of pancreas Langerhans islets. It plays an important role in glucose homeostasis which is its primary role. In addition to primary effect, it promotes a variety of other cellular events such as glycogen synthesis, lipid metabolism, regulation of ion and amino acid transport, gene transcription mRNA turnover, synthesis and degradation of proteins, and DNA synthesis. It has a key role in cell metabolism, cell survival, cellular growth, proliferation, and differentiation [1] [2] [3].
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