Insulin addition to swine semen diluted and cooled at 15 ºC

Evandro César Pereira Cunha,Luis David Solis Murgas,Márcio Gilberto Zangeronimo,Bárbara Azevedo Pereira,Luiz Gustavo Pessoa Rocha,Douglas Evangelista Braga,Bruno Generoso Faria,Luciano José Pereira

doi:10.1590/s1516-35982012000400031

Evandro César Pereira Cunha, Luis David Solis Murgas + Show 6 more

Open Access

https://doi.org/10.1590/s1516-35982012000400031

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Abstract

The objective of this study was to evaluate the effect of adding different doses of insulin to swine semen processed and stored at 15 oC. The experiment used sixteen ejaculates from four commercial breeding pigs, distributed in a randomized block design (ejaculate) with split plot along time (0, 24, 48 and 72 hours of storage) with four treatments (insulin levels - 0.0 4.0 8.0 and 12.0 IU per dose) and 16 repetitions. The experimental unit was made of two insemination doses of 100 mL each, with 3×109 spermatozoids. Insulin used was NPH-human, added at the time of processing the doses. The addition of insulin did not affect motility, sperm viability, the percentage of abnormal cells, the osmotic resistance or the degradation rate of motility in 120 minutes. There was a linear decrease in semen quality over storage time, regardless of insulin levels. The addition of insulin at the mentioned concentrations does not influence the quality of insemination dose in pigs.

Highlights

The production of pig meat has been steadily tracking the growth of the world population and the development of this production depends on factors such as nutrition, health, management, genetics and reproduction
Artificial insemination becomes an important factor in improving the reproductive rates of a facility by reducing the concentration of sperm insemination doses and longer storage of diluted semen, optimizing the use of sires in the squad
One of the main causes of the drop in fertility of stored boar semen is the decline of sperm metabolic energy (Ford & Waites, 1986), which is directly related to the amount of energy used

Summary

Introduction

The production of pig meat has been steadily tracking the growth of the world population and the development of this production depends on factors such as nutrition, health, management, genetics and reproduction. The determination of the sperm quality is essential, since infertility problems cause an increase of return to estrus rate and decreased birth rate, besides the reduction in average litter size (García et al, 2002). In this regard, several studies aimed at improving the quality of boar semen have been conducted (Vianna et al, 2004; Martins et al, 2009; Alvarenga et al, 2009). The biggest challenge is to know the components that directly influence the metabolism of sperm

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