Abstract
The design, construction and initial tests on a combined laser light scattering and synchrotron X-ray scattering instrument can cover studies of length scales from atomic sizes in Angstroms to microns and dynamics from microseconds to seconds are presented. In addition to static light scattering (SLS), dynamic light scattering (DLS), small angle X-ray scattering (SAXS) and wide angle X-ray diffraction (WAXD), the light scattering instrument is being developed to carry out studies in mildly turbid solutions, in the presence of multiple scattering. Three-dimensional photon cross correlation function (3D-PCCF) measurements have been introduced to couple with synchrotron X-ray scattering to study the structure, size and dynamics of macromolecules in solution.
Highlights
We describe the design, construction, and initial tests of an instrument using a combination of laser and synchrotron X-rays to investigate the structure and dynamics of macromolecular solutions or colloidal suspensions over a broad range of time and length scales
For those less familiar with PCCF, it is recommended that the readers first read 3D Photon Cross-Correlation Function (3D-PCCF) in Section 3.1.2. in order to be more familiar with the combination of PCCF and small angle X-ray scattering (SAXS)/wide angle X-ray diffraction (WAXD)
In addition to the instrumental improvements as to be described in the Experimental Section for separate measurements of PCCF and SAXS/WAXD, two further requirements must be satisfied in order to combine the two techniques together
Summary
Investigations on the changes in sizes, size distributions, and structures of bio-macromolecular aggregates in solution, such as the formation of Aβ peptides and complexes based on their interactions with inhibitors (or C-terminal fragments (CTFs) of Aβ42), are challenging issues. 2015, 16 techniques have been used to elucidate (bio-) macromolecular aggregates and their corresponding complexes, more probes that can provide fundamental information in the structure and dynamics of such systems should be useful. We describe the design, construction, and initial tests of an instrument using a combination of laser and synchrotron X-rays to investigate the structure and dynamics of macromolecular solutions or colloidal suspensions over a broad range of time and length scales. The description includes a brief summary of the rationale on choosing the specific designs for the instrumentation, as there are different pathways available
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