Instrumentation of Raman Spectrometry for Acquisition of Molecular Vibration Structure

Abstract

Determining the structure of an organic compound are mainly based on the physical and chemical properties, because molecules are so small it is so hard to determine the molecular structure of a compound. In this study, a simple spectrometer is developed through instrumentation and control to perform acquisition of the molecular vibrations and determine the Raman shift in wavenumber of a sample. Because the vibration phenomenon of a molecular bond can be investigated under the Raman spectrum, a charged coupled device (CCD) was used in the instrumentation together with a diffraction grating of 1000lines/mm. The spectrometer built using a charged coupled device (CCD) array is capable of detecting ultra-violet, visible, and near-infrared spectrum. In the near-infrared region the identifying marks of the samples is recorded with energy spectral density that describes the optical wavelength and peaks in the IR spectrum. Using the reaction in the saccharomyces cerevisiae in water, the energy spectral density is monitored and shows that 81.52% of light is absorbed by the given sample during the reaction with wavenumber of 605.75cm-1 which suggest that the molecular vibration is occurring at the fingerprint region under the vibrational spectrum, and light absorption becomes weaker as saccharomyces cerevisiae stop producing carbon dioxide in water with 8.39% but with traces of chemical compounds at 623.14cm-1 Raman shift in wavenumber.