Abstract

3D reconstruction is a challenging current topic in medical research. We perform 3D reconstructions from serial sections stained by immunohistological methods. This paper presents an immersive visualization solution to quality control (QC), inspect, and analyze such reconstructions. QC is essential to establish correct digital processing methodologies. Visual analytics, such as annotation placement, mesh painting, and classification utility, facilitates medical research insights. We propose a visualization in virtual reality (VR) for these purposes. In this manner, we advance the microanatomical research of human bone marrow and spleen. Both 3D reconstructions and original data are available in VR. Data inspection is streamlined by subtle implementation details and general immersion in VR.

Highlights

  • Visualization is a significant part of modern research

  • We present a method for controlling that 3D reconstructions tightly correspond to the original immunostained sections by directly comparing the reconstructions to the original serial sections

  • Immersive virtual reality (VR) approach for inspection, quality control (QC), and visual analytics (VA) of histological 3D reconstructions

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Summary

Introduction

Visualization is a significant part of modern research. It is important to obtain images for visualization, but to be able to grasp and correctly interpret data. We use virtual reality for quality control (QC) and visual analytics (VA) of our 3D reconstructions in medical research. Load multiple meshes corresponding to parts of the model and to switch between them. This allows for the analysis of multiple “channels” from different stainings. Representing different stainings as different meshes allows to remove any of the meshes at will, which is important to the in-depths study of the various aspects of the reconstruction.

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