Insinuation Salmonella Typhi for 23Na and 60Co Radioactive Sources

Abstract

The study investigates the effect of radioactive sources on Salmonella typhi, a gram-negative, rod-shaped bacterium that infects various cell types. Salmonella primarily spreads through contaminated food, commonly tainted by human or animal waste. In this descriptive research, 50 S. typhi samples from diverse patients at Baghdad Hospital were utilized. The method involved identifying the bacteria using Viteck2, culturing them on Nutrient broth and Nutrient agar, and exposing them to various radioactive sources: Na23 and CO60 (1 µCi and CO60 10 µCi). Petri dishes containing Nutrient agar were used for sample plating, followed by incubation for 24 hours at 37°C. The results showed that exposure to beta and gamma rays emitted by Na23 at an activity level of 10 µCi resulted in increased radiation levels with time. Without Almmonium, the radiation doses for 1, 2, and 3 hours were 9.64 mSv, 19.29 mSv, and 28.93 mSv, respectively, leading to varying viable cell counts. With Almmonium, the doses for the same periods were significantly lower, with fewer viable cells. Similarly, exposure to CO60 (1 µCi) resulted in varied radiation levels and viable cell counts, indicating a similar trend with and without Almmonium. CO60 (10 µCi) also demonstrated an increase in radiation doses over time, displaying an impact on viable cell counts. The study concludes that radiation emitted from Sodium and Cobalt radioactive sources exhibited effectiveness in eliminating S. typhi in direct proportion to increased exposure time and dose. This research highlights the potential for radioactive sources to impact the viability of Salmonella typhi cells.