Abstract
Cellulosic biomass, the most remarkable renewable source of fuels and other compounds of potential importance, needs highly complex mixture of various enzymes for its degradation. For sustainable and economical bioprocess, the availability of these enzymes in high quantities and at a low price is much warranted. The advancements in discovery of new strains, highly valuable techniques and molecular biotechnological tools have led to Pichia pastoris gaining a recognized organism status both at industrial as well as laboratory level. With numerous beneficial characteristics on its credit P. pastoris has emerged a promising host for most of heterologous proteins production. Thus, the production of fungal cellulytic enzymes is of worth to be comprehensively illustrated. There are some activators including promoters used in the expression host to increase the utility of expression system. This review summarizes the heterologous expression of fungal cellulases (Exo-glucanases, endo-glucanases (EGs) and beta-glucosidases (BGLs)) and advocates the various kinds of promoters used in P. Pastoris for expression of these enzymes and proteins. Further, concluding remarks will provide insights revealing the scope of P. pastoris as a potential expression system tool in today's modern direction of research.
Highlights
Biomass is a principal source of food, coarse food, paper, pulp as well as delivers bioenergy ~10.2% (50.3 EJ/yr) of the total worldwide primary energy [1]
The promoters used in fungi for heterologous expression of genes are broadly divided into constitutive and inducible promoters, though all arise from genes that are extremely expressed in the specified expression hosts
While the same enzymes have been expressed in P. pastoris under the methanol inducible promoter and compared both Yarrowia lipolytica and P. pastoris as an expression host system, showed that Y. lipolytica produced endoglucanase II and cellobiohydrolase II about 15 mg/l and 50 mg/l respectively, which is higher than P. pastoris proteins production ability
Summary
Biomass is a principal source of food, coarse food, paper, pulp as well as delivers bioenergy ~10.2% (50.3 EJ/yr) of the total worldwide primary energy [1]. The filamentous fungi are a prevalent source of fungal cellulases enzymes which are commonly exploited in the industry for biodegradation of biomass into useful products (i.e biofuels and chemical molecules) with a wide range of applications. Pichia pastoris (Komagataella phaffii) has emerged as an alternative host system for the heterologous expression of desiredenzymes and proteins [9]. A well-organized secretion tools and the relative simplicity of getting high dry cell weights >100 g/L through bioreactor fermentation make P. pastoris a popular alternative expression host for enzymes and proteins both at industrial and laboratory levels [11]. This review gives detail elucidation on heterologous expression of fungal cellulases (Exo-glucanases, endo-glucanases (EGs) and beta-glucosidases (BGLs)) and various kinds of promoters commonly used in P. Pastoris for enzymes and proteins expression, to reveal the scope of P. pastoris in today's modern field of research as a good expression system tool
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
