Abstract
Zein exhibited viscoelastic properties similar to those of wheat dough under appropriate conditions. To improve the characteristics of zein in wheat dough, wheat doughs with added zein (3%) were prepared under redox, salt, and acidic conditions. The incorporation of glutathione (GSH) into the zein-added dough (MGFZ) resulted in reduced viscoelasticity and increased creep strain, corresponding to a more relaxed water distribution, similar to what has been reported in wheat dough. In contrast, the salt-added dough exhibited the opposite trend, with a recovery rate reaching 70.11%. Azodicarbonamide (ADA) and acidic conditions showed a tendency to increase the viscoelasticity of MGFZ. Size exclusion high-performance liquid chromatography (SE-HPLC) demonstrated that ADA and salt induced protein aggregation. Moreover, ADA and salt induced a 2.37% and 7.47% decrease in weight loss, respectively, as detected by thermogravimetric analysis (TGA) when compared with MGFZ. The study provided insights into the effects of redox agents, salt, and acidic environments on zein-enriched wheat dough, potentially enhancing the applicability of zein in wheat-based products.
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