Insights into the stabilization of landfill by assessing the diversity and dynamic succession of bacterial community and its associated bio-metabolic process

Abstract

The distribution of bacterial community in an actual landfill was analyzed and the bioprocess involved in refuse degradation was clarified. The results showed that the degradation degree of refuse showed great differences with the landfill age, in which the contents of organic matter (OM) and total Kjeldahl nitrogen (TKN) in refuse as well as the chemical oxygen demand (COD) in leachate presented decreasing trends with increasing landfill age. The diversity of bacterial community increased first and then decreased with increasing landfill age. The main bacterial phyla involved in refuse degradation were Proteobacteria, Firmicutes and Bacteroidetes, among which, Proteobacteria had an absolute advantage with a relative abundance ranging of 66–78%. With increasing landfill age, the abundance of Firmicutes decreased gradually, while that of Bacteroidetes increased. Pseudomonas, Thiopseudomonas, Psychrobacter and Desemzia were the main genera. The distribution of bacterial community in samples with landfill ages of 0–1 and 1–3 years were greatly influenced by TKN and pH, respectively. Amino acid and carbohydrate metabolism were the main biological pathways according to the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, and the biodegradation of xenobiotics as well as terpenoids and polyketides also accounted relatively high frequencies in the landfill. These results provide a better understanding of landfill microbiology and bioprocesses for landfill stabilization.