Insights into the degradation of high-density polyethylene microplastics using microbial strains: Effect of process parameters, degradation kinetics and modeling

Abstract

The extensive distribution of microplastics and their abundance around the world has raised a global concern because of the lack of proper disposal channels as well as poor knowledge of their implications on human health. Sustainable remediation techniques are required owing to the absence of proper disposal methods. The present study explores the deterioration process of high-density polyethylene (HDPE) microplastics using various microbes along with the kinetics and modeling of the process using multiple non-linear regression models. Ten different microbial strains were used for the degradation of microplastics for a period of 30 days. Effect of process parameters on the degradation process was studied with the selected five microbial strains that presented the best degradation results. The reproducibility and efficacy of the process were tested for an extended period of 90 days. Fourier-transform infrared spectroscopy (FTIR) and field emission-scanning electron microscopy (FE-SEM) were used for the analysis of microplastics. Polymer reduction and half-life were evaluated. Pseudomonas putida achieved the maximum degradation efficiency of 12.07% followed by Rhodococcus ruber (11.36%), Pseudomonas stutzeri (8.28%), Bacillus cereus (8.26%), and Brevibacillus borstelensis (8.02%) after 90 days. Out of 14 models tested, 5 were found capable of modeling the process kinetics and based on simplicity and statistical data, Modified Michaelis-Menten model (F8; R2 = 0.97) was selected as superior to others. This study successfully establishes the potential of bioremediation of microplastics as the viable process.