Abstract
Recent advances in mathematical modeling of protein chromatography have focused on two areas: intra-particle transport and interactions with the sorbent surface. The influence of intra-particle transport has been investigated critically and new theories for intra-particle convection and diffusion have been developed. Careful modeling of so-called ‘perfusion’ chromatography has also provided insight into conventional protein chromatography. Novel experimental methods have been designed to verify or reject the above theories. The adsorption of proteins has been more rigorously investigated, and thermodynamic aspects, protein—protein interaction and the availability of binding sites have been incorporated into existing theories. Finally, algorithms for the optimization of operational variables, such as gradient shape, flow rate, and column utilization, have been incorporated into strategies for rational process design. These strategies use existing or modified mathematical models for the description of migration of a protein zone through a chromatography column.
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