Abstract
BackgroundThe Streptomyces albus J1074 strain is one of the most widely used chassis for the heterologous production of bioactive natural products. The fast growth and an efficient genetic system make this strain an attractive model for expressing cryptic biosynthetic pathways to aid drug discovery.ResultsTo improve its capabilities for the heterologous expression of biosynthetic gene clusters, the complete genomic sequence of S. albus J1074 was obtained. With a size of 6,841,649 bp, coding for 5,832 genes, its genome is the smallest within the genus streptomycetes. Genome analysis revealed a strong tendency to reduce the number of genetic duplicates. The whole transcriptomes were sequenced at different time points to identify the early metabolic switch from the exponential to the stationary phase in S. albus J1074.ConclusionsS. albus J1074 carries the smallest genome among the completely sequenced species of the genus Streptomyces. The detailed genome and transcriptome analysis discloses its capability to serve as a premium host for the heterologous production of natural products. Moreover, the genome revealed 22 additional putative secondary metabolite gene clusters that reinforce the strain’s potential for natural product synthesis.
Highlights
The Streptomyces albus J1074 strain is one of the most widely used chassis for the heterologous production of bioactive natural products
We present the complete sequence of the S. albus J1074 genome and compare it to other streptomycetes whose genomes have been sequenced
We found 842 clusters that are absent in S. albus but present in both S. coelicolor A3(2) and S. bingchenggensis
Summary
The Streptomyces albus J1074 strain is one of the most widely used chassis for the heterologous production of bioactive natural products. S. albus J1074 has long been known as a suitable host for the heterologous production of versatile secondary metabolites, ranging from marine Micromonospora secondary metabolites [1] to potent anticancer agents [2]. This strain was used to express steffimycin biosynthetic genes [3], as well as fredericamycin [4], Recent attempts to construct and improve a model host for the heterologous expression of genes encoding secondary metabolites have done so by deleting nonessential genes [9,10]. Detailed transcriptome time series of 12, 36 and 60 hours of shake-flask cultures of S. albus J1074 have been used to profile gene expression
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
