Abstract
Two bio-based polymers have been compared in this study, namely: polylactide (PLA) and polyhydroxyoctanoate (PHO). Due to their properties such as biocompatibility, and biointegrity they are considered to be valuable materials for medical purposes, i.e., creating scaffolds or wound dressings. Presented biopolymers were investigated for their impact on cellular migration strategies of mouse embryonic fibroblasts (MEF) 3T3 cell line. Advanced microscopic techniques, including confocal microscopy and immunofluorescent protocols, enabled the thorough analysis of the cell shape and migration. Application of wound healing assay combined with dedicated software allowed us to perform quantitative analysis of wound closure dynamics. The outcome of the experiments demonstrated that the wound closure dynamics for PLA differs from PHO. Single fibroblasts grown on PLA moved 1.5-fold faster, than those migrating on the PHO surface. However, when a layer of cells was considered, the wound closure was by 4.1 h faster for PHO material. The accomplished work confirms the potential of PLA and PHO as excellent candidates for medical applications, due to their properties that propagate cell migration, vitality, and proliferation—essential cell processes in the healing of damaged tissues.
Highlights
The investigation of biopolymers has been reserved for biochemists and molecular biologists for over half of a century
Since both PLA and PHA can potentially be used as a wound dressing material or absorbable surgical sutures, it was decided to use mouse embryonic fibroblasts (MEFs) as a model cell line for cytotoxicity tests because fibroblasts play an important role in wound healing processes, and are common in the body [40,41]
Migration antissue important process associated with numerous physiological phenomena such as on the surface polyesters to assess their usefulness for theitproduction of biomedical such wound healingofand tissue regeneration
Summary
The investigation of biopolymers has been reserved for biochemists and molecular biologists for over half of a century. One of the intensely studied and widely used group of biopolymers are polyesters derived from nature These are polyhydroxyalkanoates (PHAs) of bacterial origin [3], a class of optically active biodegradable polyesters [4]. They are stored inside the microbes’ cells in the form of granules, as internal carbon and energy storage compounds and part of their survival mechanism [5], which are easy to recover and purify [6]. PLA is one of the most innovative materials being actively investigated for a wide range of industrial applications This biodegradable and biocompatible polymer is a linear aliphatic thermoplastic polyester.
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