Abstract
Formation of oxidative stress in dermal fibroblasts plays crucial roles in aging processes of skin. The use of phytochemicals that can promote capacity of fibroblasts to combat oxidative stress is an attractive strategy to prevent skin aging and promote skin beauty. Centella asiatica has been used to treat multitude of diseases for centuries. Previous investigations demonstrated that extracts from C. asiatica have a broad range of beneficial activities through their antioxidant activity. Hence, the extract from this medicinal plant could be a great candidate for anti-skin-aging agent. Callus culture offers a powerful platform for sustainable, rapid and large-scale production of phytochemicals to serve extensive demands of pharmaceutical and cosmeceutical industries. Here, we demonstrated the application of callus culture of Centella asiatica to produce bioactive metabolites. The 50% ethanolic extract of callus culture has distinctive features of chemical compositions and biological profiles. Information from HPTLC-DPPH and HPLC analysis suggested that the callus extract comprises distinctive antioxidant compounds, compared with those isolated from authentic plant. Moreover, results from cell culture experiment demonstrated that callus extract possesses promising antioxidant and anti-skin-aging activities. Pre-treatment with callus extract attenuated H2O2-induced-cytotoxicity on human dermal fibroblasts. The results from RT-qPCR clearly suggested that the upregulation of cellular antioxidant enzymes appeared to be major contributor for the protective effects of callus extract against oxidative stress. Moreover, supplementation with callus extract inhibited induction of matrix metalloprotease-9 following H2O2 exposure, suggesting its potential anti-skin-aging activity. Our results demonstrate the potential utility of C. asiatica callus extract as anti-skin-aging agent in cosmeceutical preparations.
Highlights
Formation of oxidative stress in dermal fibroblasts plays crucial roles in aging processes of skin
We demonstrated the application of callus culture of C. asiatica to produce bioactive metabolites
To determine a suitable solvent for extraction process, callus culture and authentic plant of C. asiatica were extracted with series of solvents, including methanol, 95% ethanol, 70% ethanol, and 50% ethanol
Summary
Formation of oxidative stress in dermal fibroblasts plays crucial roles in aging processes of skin. Induction of oxidative stress in fibroblasts following exposure to external inducers e.g. ultraviolet r adiation[3], tobacco s moke[4], and air p ollutants[5], causes a reduction in biosynthesis of collagen as well as a promotion of degradation of collagen This aberrant homeostasis of collagen weakens structural integrity and mechanical properties of dermal skin, which eventually resulting in the emergence of aging characteristics, e.g. formation of wrinkle and loss of skin elasticity[6]. Plant cell culture represents an attractive platform for mass production of phytochemicals In this present study, we demonstrated the application of callus culture of C. asiatica to produce bioactive metabolites. Data from our study will provide an invaluable insight into potential application of C. asiatica callus extract in anti-aging cosmeceutical products
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