Abstract
Biomphalaria glabrata is an important host in the transmission of human schistosomiasis in the Caribbean and South America. Therefore, it is of interest to analyse the proteome data of Biomphalaria glabrata hemolymph to identify immunity related proteins in host-pathogen relationship. We used shotgun proteomic and bioinformatic analyses of the non-depleted and depleted [0.5 and 0.75% Trifluoroacetic acid (TFA) depletion] hemolymph of B. glabrata (LE strain). Analysis showed 148 proteins from the hemolymph. 148 were obtained from the 0.5% TFA-depleted sample. 62 proteins follow this from the 0.75% TFA-depleted sample. However, only 59 were found from non-depleted hemolymph. A number of proteins were identified from the hemolymph of this schistosomiasis snail vector linked to immunity related functions. This provides insights to the understanding of schistosome-snail interaction.
Highlights
Biomphalaria glabrata is an important host in the transmission of human schistosomiasis [1, 2], which has been reported in 78 countries, amounting to more than 240 million people infected worldwide [3]
We observed that the slide-centrifuged method employed was able to remove the hemocytes and other cells that may be present from the hemolymph completely (Figure 1)
Studied had shown that the B. glabrata hemolymph is very important since it house the circulating hemocytes and protein components which play an immunological role in the relationship with parasites such, as S. mansoni developing larval, at the cellular and molecular level [2, 11]
Summary
Biomphalaria glabrata is an important host in the transmission of human schistosomiasis [1, 2], which has been reported in 78 countries, amounting to more than 240 million people infected worldwide [3]. The knowledge on proteome composition of B. glabrata hemolymph, in which the internal defense mechanisms are impended, could provide clues in vaccine development. Proteomic analysis of Biomphalaria glabrata hemocytes have being shown [2] the proteome of the hemolymph is still unknow. The protein profile of B. glabrata hemolymph is dominated mostly by different proteoforms of hemocyanin [8]. It is of interest to analyze the proteome data of Biomphalaria glabrata hemolymph to identify immunity related proteins in host-pathogen relationship
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