Insight the roles of loosely-bound and tightly-bound extracellular polymeric substances on Cu2+, Zn2+ and Pb2+ biosorption process with Desulfovibrio vulgaris

Abstract

The aim of this study is to explore the fate and mechanism of metal cations of biosorption in the Desulfovibrio vulgaris system (including bacterial cells and secreted loosely-bound extracellular polymeric substances (LB-EPS) and tightly-bound extracellular polymeric substances (TB-EPS)). The relative contribution of EPS (TB-EPS and LB-EPS) to the adsorption of three metal cations is much greater than that of bacterial cells, and the adsorption capacity of Pb2+ on EPS (TB-EPS and LB-EPS) is much greater than that of Cu2+ and Zn2+ (Pb2+ > Cu2+ > Zn2+). The order of absorption capacity was as follows: LB-EPS > TB-EPS > bacterial cells, the adsorption contribution of EPS (including TB-EPS and LB-EPS) to Cu2+, Zn2+ and Pb2+ accounted for total adsorption capacity was 82%, 83% and 86%, respectively. It was suggested that LB-EPS was the first reaction barrier of immobilization metal cations before metal cations was able to pass through EPS and react with cells. The adsorption process was dominated by complexation and electrostatic interaction. The three-dimensional excitation-emission matrix (3D-EEM) identified two main fluorescence peaks of the aromatic and tryptophan protein-like substances in EPS. According to the synchronous fluorescence spectra, the tryptophan protein-like substances were gradually quenched with increased metal cations concentrations, which the quencher mechanism is dynamic quenching. The findings of this work are significant to reveal the fate of Cu2+, Zn2+ and Pb2+ during its sorption process onto Desulfovibrio vulgaris, and provide useful information of the interaction between Desulfovibrio vulgaris and its secreted EPS with metal cations.