Abstract
BackgroundPyropia haitanensis, distributes in the intertidal zone, can tolerate water losses exceeding 90%. However, the mechanisms enabling P. haitanensis to survive harsh conditions remain uncharacterized. To elucidate the mechanism underlying P. haitanensis desiccation tolerance, we completed an integrated analysis of its transcriptome and proteome as well as transgenic Chlamydomonas reinhardtii carrying a P. haitanensis gene.ResultsP. haitanensis rapidly adjusted its physiological activities to compensate for water losses up to 60%, after which, photosynthesis, antioxidant systems, chaperones, and cytoskeleton were activated to response to severe desiccation stress. The integrative analysis suggested that transketolase (TKL) was affected by all desiccation treatments. Transgenic C. reinhardtii cells overexpressed PhTKL grew better than the wild-type cells in response to osmotic stress.ConclusionP. haitanensis quickly establishes acclimatory homeostasis regarding its transcriptome and proteome to ensure its thalli can recover after being rehydrated. Additionally, PhTKL is vital for P. haitanensis desiccation tolerance. The present data may provide new insights for the breeding of algae and plants exhibiting enhanced desiccation tolerance.
Highlights
Pyropia haitanensis, distributes in the intertidal zone, can tolerate water losses exceeding 90%
Principal component analysis of Pyropia haitanensis transcriptomic and proteomic data Details regarding the de novo transcriptome assembly and functional annotations are provided in Additional file 1: Table S3
The isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic analysis of all the P. haitanensis samples resulted in 149,413 spectra
Summary
Distributes in the intertidal zone, can tolerate water losses exceeding 90%. The annual production of Pyropia species (many of which were formerly Porphyra species) is currently valued at nearly $950 million, with the highest commercial value per unit mass (almost $523 per wet metric ton) among all aquaculture seaweed species [2]. These species are widely used as sources of food, fertilizer, medicine, and chemicals [1]. Pyropia species may contribute to the production of “blue carbon” and represent a potentially useful source of biofuel. Originally identified in Fujian Province in China, is a typical warm temperate zone species, and it accounts for 75% of the total production of Pyropia species in China [4]
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