Abstract
Salidroside is the important active ingredient of Rhodiola species, which shows a wide range of pharmacological activities such as antioxidative stress, anti-inflammation, and antiliver fibrosis. In this paper, we aimed to study the protective effect and mechanism of salidroside against H2O2-induced oxidative damage in H9C2 cells by determining cell proliferation rate, intracellular reactive oxygen species (ROS) level, antioxidant enzyme activities, and the expression of apoptosis-related proteins. The results showed that salidroside significantly alleviated cell growth inhibition induced by H2O2 treatment in H9C2 cells, decreased the levels of intracellular ROS and malondialdehyde (MDA), and increased the activity of superoxide dismutase (SOD) and catalase (CAT); meanwhile, salidroside upregulated the expression of Bcl-2 while downregulated the expression of Bax, p53, and caspase-3 in H2O2-treated H9C2 cells. Furthermore, the antiapoptotic effect of salidroside was almost eliminated by the knockdown of Bcl-2. In the further exploration, the Bcl-2 expression was decreased by the p53 overexpression and increased by p53 knockdown in H2O2-treated H9C2 cells. Consequently, salidroside could protect H9C2 cells against H2O2-induced oxidative damage, and the underlying mechanism may be related to scavenging intracellular ROS, increasing the activities of intracellular antioxidant enzymes and inhibiting the expression of apoptosis-related proteins.
Highlights
Reactive oxygen species (ROS) is considered as the second messenger in cells, which implicates in the processes of cell proliferation, differentiation, and apoptosis [1]
H2O2 treatment could significantly induce the increase of intracellular ROS level in H9C2 cells, while the ROS level was significantly reduced by salidroside treatment in a dosedependent manner (Figure 1(d))
These results indicated that salidroside can improve oxidative stress injury induced by H2O2 in H9C2 cells
Summary
Reactive oxygen species (ROS) is considered as the second messenger in cells, which implicates in the processes of cell proliferation, differentiation, and apoptosis [1]. A large number of publications have shown that oxidative stress-induced cardiomyocyte apoptosis plays an important role in the development and progression of cardiovascular diseases [3,4,5,6] In this case, the search for new natural compounds which exert effects on inhibiting oxidative stress and reducing cardiomyocyte apoptosis is considered a promising strategy for the improvement of cardiovascular disease. The search for new natural compounds which exert effects on inhibiting oxidative stress and reducing cardiomyocyte apoptosis is considered a promising strategy for the improvement of cardiovascular disease Many natural ingredients such as flavonoids [7, 8], polyphenols [9, 10], and alkaloids [11] have been reported to have antioxidant or protective properties, and they may be valuable for the treatment of myocardial injury
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