Abstract
An insertional mutagenesis screen identifies pathogenicity-related genes in the plant fungal pathogen Fusarium oxysporum.
Highlights
Fusarium oxysporum f. sp. lycopersici is the causal agent of vascular wilt disease in tomato
Identification of pathogenicity mutants from a collection of F. oxysporum transformants A collection of 10,290 transformants was generated through Agrobacterium-mediated transformation using the transfer DNA (T-DNA) of pPK2hphgfp as insertional mutagen (Figure 1)
The pathogenicity mutants were classified according to severity of pathogenicity loss, based on the average disease index
Summary
Fusarium oxysporum f. sp. lycopersici is the causal agent of vascular wilt disease in tomato. The process of vascular infection has been studied using light, fluorescence and electron microscopy and can be divided into several steps: root recognition, root surface attachment and colonization, penetration of the root cortex, and hyphal proliferation within the xylem vessels. This hyphal proliferation in vessels causes characteristic disease symptoms, such as vein clearing, leaf epinasty, wilt and defolation, eventually leading to death of the host plant. At this stage, F. oxysporum invades the parenchymatous tissue and starts sporulating on the plant surface, thereby completing its pathogenic life cycle [6]
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