Insight into the CSA tensors of nucleobase carbons in RNA polynucleotides from solution measurements of residual CSA: Towards new long-range orientational constraints

Abstract

Using residual chemical shift anisotropies (RCSAs) measured in a weakly aligned stem–loop RNA, we examined the carbon chemical shift anisotropy (CSA) tensors of nucleobase adenine C2, pyrimidine C5 and C6, and purine C8. The differences between the measured RCSAs and the values back-calculated using three nucleobase carbon CSA sets [D. Stueber, D.M. Grant, 13C and (15)N chemical shift tensors in adenosine, guanosine dihydrate, 2′-deoxythymidine, and cytidine, J. Am. Chem. Soc. 124 (2002) 10539–10551; D. Sitkoff, D.A. Case, Theories of chemical shift anisotropies in proteins and nucleic acids, Prog. NMR Spectrosc. 32 (1998) 165–190; R. Fiala, J. Czernek, V. Sklenar, Transverse relaxation optimized triple-resonance NMR experiments for nucleic acids, J. Biomol. NMR 16 (2000) 291–302] reported previously for mononucleotides (1.4 Hz) is significantly smaller than the predicted RCSA range (−10–10 Hz) but remains larger than the RCSA measurement uncertainty (0.8 Hz). Fitting of the traceless principal CSA values to the measured RCSAs using a grid search procedure yields a cytosine C5 CSA magnitude ( CSA a = ( 3 / 2 · ( δ 11 2 + δ 22 2 + δ 33 2 ) ) 1 / 2 = 173 ± 21 ppm ) , which is significantly higher than the reported mononucleotide values (131–138 ppm) and a guanine C8 CSA a (148 ± 13 ppm) that is in very good agreement with the mononucleotide value reported by solid-state NMR [134 ppm, D. Stueber, D.M. Grant, 13C and (15)N chemical shift tensors in adenosine, guanosine dihydrate, 2′-deoxythymidine, and cytidine, J. Am. Chem. Soc. 124 (2002) 10539–10551]. Owing to a unique sensitivity to directions normal to the base plane, the RCSAs can be translated into useful long-range orientational constraints for RNA structure determination even after allowing for substantial uncertainty in the nucleobase carbon CSA tensors.