Insight into microbial degradation of hexabromocyclododecane (HBCD) in lake sediments under different hydrodynamic conditions

Abstract

Hexabromocyclododecane (HBCD), an emerging persistent organic pollutant, has been widely detected in aquatic ecosystems with various hydrodynamic conditions, however, the effects of hydrodynamic changes on microbial degradation of HBCD in aquatic sediment remains unclear. Here, we conducted an annular flume experiment to characterize variation in HBCD removal from contaminated sediment under three hydrodynamic conditions with different flow velocities, as well as clarify the underlying microbial mechanisms. We detected significant HBCD removal and bromine ion generation in all contaminated sediments, and microbial reduction debromination was an important process for HBCD removal. At the end of the 49-day experiment, both HBCD removal percentage and the bromine ion concentration were significantly lower under dynamic water condition with higher sediment redox potential, compared with static water conditions. The dynamic water conditions resulted a relatively high sediment redox potential and decreased the iron reduction rate and the abundance of organohalide-respiring bacteria (OHRB) in the genera Geobatcer, Dehalogenimonas, Dehalobacter, and Dehalococcoide, which reduced the microbial degradation of HBCD in contaminated sediments. The community composition of both total bacteria and OHRB also differed significantly among hydrodynamic conditions. Some bacterial groups with HBCD degradation abilities such as Pseudomonas and Sulfuricurvum were less abundant under dynamic water conditions, and the HBCD degradation efficiencies were lower. These findings enhance our understanding of the bioremediation potential of HBCD-contaminated sediments in different hydrodynamic areas.