Abstract
A new suite of 2D FOSY experiments focuses onto one coupled nuclear spin system at a time, that is, a post-translational modification or a structural hotspot in general, with three to five known frequencies. With higher sensitivity and versatility than achievable by traditional experiments, FOSY solves the spectral dispersion problem and obtains only the relevant local NMR signal assignment in a few hours as demonstrated by Vladislav Y. Orekhov and co-workers in their Communication on page 23540 for two phosphorylation sites in the 441-residue IDP Tau.
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