Inside Back Cover: High-Throughput Screening for Terpene-Synthase-Cyclization Activity and Directed Evolution of a Terpene Synthase (Angew. Chem. Int. Ed. 21/2013)

Abstract

Active terpene synthase mutants are generally identified by using lower-throughput GC-based methods. In their Communication on page 5571 ff., F. H. Arnold and co-workers describe a terpene synthase substrate that enables cyclization activity to be screened in a high-throughput manner. The screening protocol allows the identification of active mutants by the brilliant purple color that these mutants generate in the assay.