Abstract
Inside Alzheimer brain with CLARITY: senile plaques, neurofibrillary tangles and axons in 3-D.
Highlights
We noticed that the results were improved if ScaleA2 [8] was used to mount the block after CLARITY
After a blocking stage (10 % normal mouse serum), the tissues were incubated with biotin-labelled 4G8 anti-amyloid β (Aβ) antibody (Covance), revealed with DyLight 488-labelled streptavidin (KPL, Eurobio, France)
Triple staining (Aβ, tau and neurofilament) was performed: the block was incubated with anti-tau B19 and anti-neurofilament M0762 antibodies revealed with the secondary Alexa Fluor® 488 goat antirabbit and Alexa Fluor® 568 goat anti-mouse antibodies
Summary
We noticed that the results were improved if ScaleA2 [8] was used to mount the block after CLARITY. The blocks were immunostained with a rabbit polyclonal anti-tau B19 antibody [3] and a mouse monoclonal anti-Aβ 4G8 antibody (Covance). For Aβ and neurofilament double immunohistochemistry, the tissues were first incubated with mouse monoclonal anti-neurofilament antibody (M0762 Dako) and with Alexa Fluor® 568 goat anti-mouse antibody.
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