Insecticides resistance and detoxification enzymes activity changes in brown planthopper, Nilaparvata lugens in Guizhou Province

Abstract

The brown planthopper (BPH), Nilaparvata lugens (Homoptera: Delphacidae), is a kind of seriously pest of rice crops throughout Asia and effective control is essential for economical crop production. Insecticides Abamectin, Imidacloprid, Chlorantraniliprole, Phoxim and Chlorpyrifos have been broadly used to control the brown planthopper for recent years. To investigate the resistance development of the BPH in Guizhou Province, we collected BPH from 5 sites (Panxian, Jinping, Sandu, Daozhen and Huaxi) in Guizhou Province June–August 2014. Their responses to two diagnostic doses of 5 insecticides (LD50 and detoxification enzyme activity) were examined. The results showed that LD50 value for Chlorantraniliprole in Jinping and Huaxi BPH population were 199.810 and 282.082 μg/g higher than imidacloprid, phoxim and chlorpyrifos. LD50 value for Imidacloprid in Jinping population was 3.957 μg/g lower than other BPH populations for 4 insecticides. The GSTs activity is significantly higher with Abamectin, Chlorpyrifos and Phoxim treatments. AchE activity is higher with Chlorantraniliprole and Imidacloprid, but lowest among three enzymes with Phoxim and Chlorpyrifos. The data demonstrate the development and spread of multiple resistance in N. lugens in Guizhou Province and support reports of reduced field efficacy of imidacloprid.