Insecticide resistance management of Bemisia tabaci (Hemiptera: Aleyrodidae) in Australian cotton - pyriproxyfen, spirotetramat and buprofezin.

Abstract

Bemisia tabaci is a globally significant agricultural pest including in Australia, where it exhibits resistance to numerous insecticides. With a recent label change, buprofezin (group 16), is now used for whitefly management in Australia. This study investigated resistance to pyriproxyfen (group 7C), spirotetramat (group 23) and buprofezin using bioassays and available molecular markers. Bioassay and selection testing of B.tabaci populations detected resistance to pyriproxyfen with resistance ratios ranging from 4.1 to 56. Resistance to spirotetramat was detected using bioassay, selection testing and sequencing techniques. In populations collected from cotton, the A2083V mutation was detected in three populations of 85 tested, at frequencies ≤4.1%, whereas in limited surveillance of populations from an intensive horticultural region the frequency was ≥75.8%. The baseline susceptibility of B.tabaci to buprofezin was determined from populations tested from 2019 to 2020, in which LC50 values ranged from 0.61 to 10.75 mg L-1 . From the bioassay data, a discriminating dose of 200 mg L-1 was developed. Recent surveillance of 16 populations detected no evidence of resistance with 100% mortality recorded at doses ≤32 mg L-1 . A cross-resistance study found no conclusive evidence of resistance to buprofezin in populations with high resistance to pyriproxyfen or spirotetramat. In Australian cotton, B.tabaci pest management is challenged by ongoing resistance to pyriproxyfen, while resistance to spirotetramat is an emerging issue. The addition of buprofezin provides a new mode-of-action for whitefly pest management, which will strengthen the existing insecticide resistance management strategy. © 2023 Commonwealth of Australia. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.