Abstract
In this study, bifenthrin (Maxxthor SC, Ensystex Australasia Pty Ltd), imidacloprid (Prothor SC, Ensystex Australasia Pty Ltd) and fipronil (Regent®50SC, Bayer) were applied on the natural infest manures according to the manufacturer rate during a broiler breeding cycle. Solvent direct-immersion extraction (SDIE) was used in detecting the target compound and later, quantification of the insecticide residues in field condition was investigated. The samples were prior cleaned up by solid-phase extraction (SPE) and analysed by Ultra-Performance Liquid Chromatography (UPLC) – photodiode array (PDA) system. In the field trial, three insecticides were showed accumulation during the broiler breeding period and it is suggested that they acted as adulticides when applied on the poultry manures, this is supported by the significant correlation between the increment of insecticide residues to the reduction percentage of adult flies (<0.05). Fipronil showed significantly greater reduction on the adult fly compared to the other insecticides, in which the reduction rate compared to control population at the end of the broiler breeding period; fipronil, imidaclopril and bifenthrin reduced 51.51%, 28.30% and 30.84% of adult flies, respectively.
Highlights
Insecticides application is a common approach in poultry unit of Malaysia for controlling the population of the major pest, house fly, Musca domestica L (Diptera; Muscidae) (Ong et al 2015)
The effectiveness of the insecticide residues in poultry manures towards the house fly are vague due to the limited reported residues detection methods. Some standard organisation such as Environment Protection Agency (EPA), America Standard Testing Methods (ASTM) and America Official Analytical Chemists (AOAC) propose some standard methods for study the insecticide residues, but these methods are focusing on soil, water, agricultural products or food (AOAC method 2007, ASTM method E1527, 2013), excluding poultry manures
The insecticides that were used in this study was based on the information provided by Department of Veterinary Services-DVS that the insecticides had been used by the poultry farms of Perak, Malaysia in 2013
Summary
Insecticides application is a common approach in poultry unit of Malaysia for controlling the population of the major pest, house fly, Musca domestica L (Diptera; Muscidae) (Ong et al 2015). Some standard organisation such as Environment Protection Agency (EPA), America Standard Testing Methods (ASTM) and America Official Analytical Chemists (AOAC) propose some standard methods for study the insecticide residues, but these methods are focusing on soil, water, agricultural products or food (AOAC method 2007, ASTM method E1527, 2013), excluding poultry manures. In this study, the residues of three insecticide; bifenthrin, imidacloprid and fipronil were prior studied using solvent direct-immersion extraction (SDIE) in extracting the residues in poultry manures and subsequently, quantified using Ultra-Performance Liquid Chromatography (UPLC) compared the efficacy infield. The study aim to study the residues efficacy in controlling house fly on poultry farm Malaysia
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